Plasma membrane microdomains from hybrid aspen cells are involved in cell wall polysaccharide biosynthesis.
Identifieur interne : 003523 ( Main/Exploration ); précédent : 003522; suivant : 003524Plasma membrane microdomains from hybrid aspen cells are involved in cell wall polysaccharide biosynthesis.
Auteurs : Laurence Bessueille [France] ; Nicolas Sindt ; Michel Guichardant ; Soraya Djerbi ; Tuula T. Teeri ; Vincent BuloneSource :
- The Biochemical journal [ 1470-8728 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Polyosides.
- composition chimique : Microdomaines membranaires.
- cytologie : Arbres.
- enzymologie : Microdomaines membranaires.
- métabolisme : Paroi cellulaire.
- physiologie : Microdomaines membranaires.
- Cellules hybrides, Glucosyltransferases, Octoxinol.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Polysaccharides.
- chemical : Glucosyltransferases, Octoxynol.
- chemistry : Membrane Microdomains.
- cytology : Trees.
- enzymology : Membrane Microdomains.
- metabolism : Cell Wall.
- physiology : Membrane Microdomains.
- Hybrid Cells.
Abstract
Detergent-resistant plasma membrane microdomains [DRMs (detergent-resistant membranes)] were isolated recently from several plant species. As for animal cells, a large range of cellular functions, such as signal transduction, endocytosis and protein trafficking, have been attributed to plant lipid rafts and DRMs. The data available are essentially based on proteomics and more approaches need to be undertaken to elucidate the precise function of individual populations of DRMs in plants. We report here the first isolation of DRMs from purified plasma membranes of a tree species, the hybrid aspen Populus tremula x tremuloides, and their biochemical characterization. Plasma membranes were solubilized with Triton X-100 and the resulting DRMs were isolated by flotation in sucrose density gradients. The DRMs were enriched in sterols, sphingolipids and glycosylphosphatidylinositol-anchored proteins and thus exhibited similar properties to DRMs from other species. However, they contained key carbohydrate synthases involved in cell wall polysaccharide biosynthesis, namely callose [(1-->3)-beta-D-glucan] and cellulose synthases. The association of these enzymes with DRMs was demonstrated using specific glucan synthase assays and antibodies, as well as biochemical and chemical approaches for the characterization of the polysaccharides synthesized in vitro by the isolated DRMs. More than 70% of the total glucan synthase activities present in the original plasma membranes was associated with the DRM fraction. In addition to shedding light on the lipid environment of callose and cellulose synthases, our results demonstrate the involvement of DRMs in the biosynthesis of important cell wall polysaccharides. This novel concept suggests a function of plant membrane microdomains in cell growth and morphogenesis.
DOI: 10.1042/BJ20082117
PubMed: 19216717
Affiliations:
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Teeri</name></author><author><name sortKey="Bulone, Vincent" sort="Bulone, Vincent" uniqKey="Bulone V" first="Vincent" last="Bulone">Vincent Bulone</name></author></analytic><series><title level="j">The Biochemical journal</title><idno type="eISSN">1470-8728</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Glucosyltransferases (MeSH)</term><term>Hybrid Cells (MeSH)</term><term>Membrane Microdomains (chemistry)</term><term>Membrane Microdomains (enzymology)</term><term>Membrane Microdomains (physiology)</term><term>Octoxynol (MeSH)</term><term>Polysaccharides (biosynthesis)</term><term>Trees (cytology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (cytologie)</term><term>Cellules hybrides (MeSH)</term><term>Glucosyltransferases (MeSH)</term><term>Microdomaines membranaires (composition chimique)</term><term>Microdomaines membranaires (enzymologie)</term><term>Microdomaines membranaires (physiologie)</term><term>Octoxinol (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Polyosides (biosynthèse)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glucosyltransferases</term><term>Octoxynol</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Membrane Microdomains</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Microdomaines membranaires</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Arbres</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Microdomaines membranaires</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Membrane Microdomains</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Microdomaines membranaires</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Membrane Microdomains</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hybrid Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules hybrides</term><term>Glucosyltransferases</term><term>Octoxinol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Detergent-resistant plasma membrane microdomains [DRMs (detergent-resistant membranes)] were isolated recently from several plant species. As for animal cells, a large range of cellular functions, such as signal transduction, endocytosis and protein trafficking, have been attributed to plant lipid rafts and DRMs. The data available are essentially based on proteomics and more approaches need to be undertaken to elucidate the precise function of individual populations of DRMs in plants. We report here the first isolation of DRMs from purified plasma membranes of a tree species, the hybrid aspen Populus tremula x tremuloides, and their biochemical characterization. Plasma membranes were solubilized with Triton X-100 and the resulting DRMs were isolated by flotation in sucrose density gradients. The DRMs were enriched in sterols, sphingolipids and glycosylphosphatidylinositol-anchored proteins and thus exhibited similar properties to DRMs from other species. However, they contained key carbohydrate synthases involved in cell wall polysaccharide biosynthesis, namely callose [(1-->3)-beta-D-glucan] and cellulose synthases. The association of these enzymes with DRMs was demonstrated using specific glucan synthase assays and antibodies, as well as biochemical and chemical approaches for the characterization of the polysaccharides synthesized in vitro by the isolated DRMs. More than 70% of the total glucan synthase activities present in the original plasma membranes was associated with the DRM fraction. In addition to shedding light on the lipid environment of callose and cellulose synthases, our results demonstrate the involvement of DRMs in the biosynthesis of important cell wall polysaccharides. This novel concept suggests a function of plant membrane microdomains in cell growth and morphogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19216717</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>20</Day></DateCompleted><DateRevised><Year>2009</Year><Month>04</Month><Day>22</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1470-8728</ISSN><JournalIssue CitedMedium="Internet"><Volume>420</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Apr</Month><Day>28</Day></PubDate></JournalIssue><Title>The Biochemical journal</Title><ISOAbbreviation>Biochem J</ISOAbbreviation></Journal><ArticleTitle>Plasma membrane microdomains from hybrid aspen cells are involved in cell wall polysaccharide biosynthesis.</ArticleTitle><Pagination><MedlinePgn>93-103</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1042/BJ20082117</ELocationID><Abstract><AbstractText>Detergent-resistant plasma membrane microdomains [DRMs (detergent-resistant membranes)] were isolated recently from several plant species. As for animal cells, a large range of cellular functions, such as signal transduction, endocytosis and protein trafficking, have been attributed to plant lipid rafts and DRMs. The data available are essentially based on proteomics and more approaches need to be undertaken to elucidate the precise function of individual populations of DRMs in plants. We report here the first isolation of DRMs from purified plasma membranes of a tree species, the hybrid aspen Populus tremula x tremuloides, and their biochemical characterization. Plasma membranes were solubilized with Triton X-100 and the resulting DRMs were isolated by flotation in sucrose density gradients. The DRMs were enriched in sterols, sphingolipids and glycosylphosphatidylinositol-anchored proteins and thus exhibited similar properties to DRMs from other species. However, they contained key carbohydrate synthases involved in cell wall polysaccharide biosynthesis, namely callose [(1-->3)-beta-D-glucan] and cellulose synthases. The association of these enzymes with DRMs was demonstrated using specific glucan synthase assays and antibodies, as well as biochemical and chemical approaches for the characterization of the polysaccharides synthesized in vitro by the isolated DRMs. More than 70% of the total glucan synthase activities present in the original plasma membranes was associated with the DRM fraction. In addition to shedding light on the lipid environment of callose and cellulose synthases, our results demonstrate the involvement of DRMs in the biosynthesis of important cell wall polysaccharides. This novel concept suggests a function of plant membrane microdomains in cell growth and morphogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bessueille</LastName><ForeName>Laurence</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Organisation et Dynamique des Membranes Biologiques, UMR CNRS (Centre National de la Recherche Scientifique) 5246, Université Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sindt</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Guichardant</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Djerbi</LastName><ForeName>Soraya</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Teeri</LastName><ForeName>Tuula T</ForeName><Initials>TT</Initials></Author><Author ValidYN="Y"><LastName>Bulone</LastName><ForeName>Vincent</ForeName><Initials>V</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>04</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biochem J</MedlineTA><NlmUniqueID>2984726R</NlmUniqueID><ISSNLinking>0264-6021</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-93-1</RegistryNumber><NameOfSubstance UI="D017830">Octoxynol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C478648">cellulose synthase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C040232">glucan synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006822" MajorTopicYN="N">Hybrid Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021962" MajorTopicYN="N">Membrane Microdomains</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017830" MajorTopicYN="N">Octoxynol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>2</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>2</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19216717</ArticleId><ArticleId IdType="pii">BJ20082117</ArticleId><ArticleId IdType="doi">10.1042/BJ20082117</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Villeurbanne</li></settlement></list><tree><noCountry><name sortKey="Bulone, Vincent" sort="Bulone, Vincent" uniqKey="Bulone V" first="Vincent" last="Bulone">Vincent Bulone</name><name sortKey="Djerbi, Soraya" sort="Djerbi, Soraya" uniqKey="Djerbi S" first="Soraya" last="Djerbi">Soraya Djerbi</name><name sortKey="Guichardant, Michel" sort="Guichardant, Michel" uniqKey="Guichardant M" first="Michel" last="Guichardant">Michel Guichardant</name><name sortKey="Sindt, Nicolas" sort="Sindt, Nicolas" uniqKey="Sindt N" first="Nicolas" last="Sindt">Nicolas Sindt</name><name sortKey="Teeri, Tuula T" sort="Teeri, Tuula T" uniqKey="Teeri T" first="Tuula T" last="Teeri">Tuula T. Teeri</name></noCountry><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Bessueille, Laurence" sort="Bessueille, Laurence" uniqKey="Bessueille L" first="Laurence" last="Bessueille">Laurence Bessueille</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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